G-2028

Molecular Detection of Toxoplasma gondii in Spontaneous Abortion Tissues: Insights from Birjand City (2022)

 Negar Nashat ¹ ℗, Peyman Bagherizadeh ¹, Amir Tavakoli Kareshk ² ©, Davood Javanmard ², Mahmoodreza Behravan ²   

 
Abstract

Introduction: Toxoplasmosis is a common infection of humans and animals, the incidence of which depends on geographical conditions, dietary habits and contact with animals. Also, considering the life-threatening complications of this parasite on the fetus during pregnancy, the importance of its investigation is doubled. Therefore, this study aimed to investigate the molecular characteristics of the Toxoplasma gondii parasite in tissue samples of spontaneous abortion cases in Birjand city in 2022. Methods and Materials: This descriptive-analytical study was conducted on 100 samples collected from women with a history of spontaneous abortion referred to Vali Asr Hospital (Aj) in Birjand city. Demographic and obstetric information as well as information related to fertility issues were obtained from the patients. Then, an examination was performed on the placenta and PCR to diagnose Toxoplasma infection. After collection, the data were analyzed using SPSS software and a significance level of 0.05. This study was approved by the Ethics Code IR.BUMS.REC.1402.208. Result: The results showed that 0.8% of the samples were positive for Toxoplasma gondii. There was no significant association between age, history of preterm delivery, type of delivery, smoking, place of residence, contact with domestic animals, and vaginal delivery with infection (P0.05). Conclusion and Discussion: Considering the reproductive age of women, it seems that the use of health education methods and increasing health awareness through public media can reduce the burden of infection and irreversible complications in newborns, and reduce the heavy costs associated with the treatment, care, and education of people with disabilities due to congenital toxoplasmosis. In addition, it is necessary to perform toxoplasmosis screening as a routine serological test for all women in all rural health centers and urban households, and to identify people without immunity to toxoplasmosis, and by providing the necessary guidance and education, the dangerous consequences of this disease can be greatly reduced.

Keywords: Toxoplasma gondii, Spontaneous Abortion, PCR, Birjand